One histological hallmark of neuropathies is loss of intraepidermal nerve fibers, as shown by skin punch biopsy from the distal and proximal leg. While the procedure is fast and simple, even small but missteps may lead to clinically relevant misinterpretations. Following standardised guidelines for processing and analysing skin samples should be mandatory to ensure accurate data. Even so, the literature is filled with heterogeneous and erroneous methodology, making comparison between studies difficult and, in worst cases, leading to inaccurate findings. Advance in methodology has carried skin biopsies far beyond the mere determination of nerve fiber density. It is, for example, possible to distinguish between somatosensory and autonomic nerve fibres, assess sweat gland innervation, quantify morphological abnormalities of the nerve fibers such as axonal swellings, and identify nerve fiber subtypes. Skin punch biopsy also allows the investigation of other cells such as keratinocytes, Langerhans cells, inflammatory cells, glia cells and many more.

This interactive workshop will teach the audience how to accurately obtain, process, and analyse skin punch biopsies for clinical and research purposes using short presentations, live demonstrations, pre-recorded videos, and on-stage microscopy.

Attendance at this workshop requires registration.  Fee:  $99 USD